English translation for "random primer"
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- 随机引物
Related Translations:
- Example Sentences:
| 1. | Three natural population of l . sagitta , add up to 62 individual , with 17 random primers , 128 polymorphic loci
箭叶橐吾的三个自然居群，总计62个个体，使用17个随机引物，共扩增出128个多态位点。 | | 2. | Five natural population of l . virgaurea , add up to 100 individual , with 17 random primers , 132 polymorphic loci
黄帚橐吾的五个自然居群，总计100个个体，使用17个随机引物，共扩增出132个多态位点。 | | 3. | Rapd technique was first used to analyze the phylogenetic relationships of 13 species or varieties in pueraria dc complex . seventy - two random primers were tested , among which 18 chosen primers generated clear polymorphic bands
从72个随机引物中筛选出了18个引物在13个不同的种和类型的葛资源中具有rapd多态性。 | | 4. | Using 20 out of 40 decamer random primers , random amplified polymorphic dna ( rapd ) was applied to analyze the diversity of 48 individuals of p . polyactis from five sampling areas in the yellow sea and the east china sea
研究主要包括如下三个部分：一、小黄鱼的遗传多样性分析了采自黄海和东海5个取样海区共计4吕个小黄鱼个体的随机扩增dna多态性。 | | 5. | Twelve primers selected from 100 random primers amplified 254 frangments in 14 species of cymbidium , in which 243 segments are polymorphic . the rate of polymorphism was 95 . 7 % . and all the segments were ranging from 200bp to 2200bp
从100个随机引物中筛选出12个随机引物，对兰属植物14种材料进行随机扩增，共得到254条带，多态性带有243条，多态性百分率为95 . 7 。 | | 6. | Finally , the variant line tc243 - 2 , was analyzed by rapd - pcr amplification with 100 random primers . the results suggested that phenotypic variation came from exogenous dna transformation rather than exogenous pollen contamination . a mutation corre
用100条随机引物对tc243一株系的rapd一pcr扩增结果表明该株系的变异不是来自外源花粉的污染，同时发现了特异条带5270 _ 1600对应的变异在tz一ts代能连续稳定遗传。 | | 7. | Random amplified polymorphic dna ( rapd ) analysis demonstrated that 16 random primers produced 129 loci , obtaining 128 polymorphic loci in 7 populations from different geographical locations . the total percentage of polymorphism was 99 . 22 % , which showed s . krylovii had abundant genetic diversity
采用rapd方法对不同地理种群的研究表明， 16个引物共扩增出129个位点，其中多态位点128个，多态位点百分率是99 . 22 ，说明克氏针茅具有较高的遗传多样性。 | | 8. | Lastly by using the technique of dot blot hybridization , the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer . the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe . by comparing the diagnostic ways of nucleotide probe and fc , the technique of nucleotide probe were proved to have high sensitivity and speci fi city
最后，用地高辛随机引物法标记成momp基因核酸探针，斑点杂交检测衣原体基因组dna ，灵敏度可达10pg ，且不能检出其它病原体的核酸。将核酸探针法与补体结合反应法对衣原体感染的诊断进行比较，初步证明该探针具有较高的敏感性与较强的特异性。 | | 9. | Similarity coefficient and genetic distances between two arbitrary individuals were caculated according to the data of experiments , the pedigree was constructed by two means ( they were upgma and parsimony ) , and genetic diversity was discussedbased on selecting the best conditions of pcr with opg and opa primers from operon company , we obtained 163 bands using 24 random primers . in other words , 163 sites in crested ibis " genome were detected . the bands from 23 primers showed polymorphism . minimurn of polymorphic ratio was 0 , maximum was 85 . 71 % , and average was 48 . 99 % . in the population which was composed by 37 individuals , most of genetic distances between two arbitrary individuals were less than 0 . 1 . the results showed that the degree of similarity among crested ibises was high while genetic diversity in the population was low accordingly
我们在对operon公司opg系列和opa系列引物进行优化筛选的基础上，用24条随机引物共扩增出163条谱带，即检测了朱?基因组中的163个位点，其中23条引物的扩增结果具有多态性；谱带的多态比率最小为0 ，最大为85 . 71 ，平均为48 . 99 ；在由37只朱?组成的种群中，任意两个个体之间的遗传距离绝大多数在0 . 1以下。这些结果表明，目前朱?个体间的相似程度很高，种群的遗传多样性水平较低。 | | 10. | Abstract : to decrease nonspecific products and obtain clear rapd patterns , 18 10 - mer random primers were tested at different annealing temperatures . the results indicated that all the amplification can be performed when the annealing temperature is in the range of 40 ~ 50 . there were a few primers with which the amplification was still performed when the annealing temperature is above 60 . by using high annealing temperatures , some primers which produce more nonspecific product at the annealing temperatures of 35 ~ 38 could generate reproducible and distinct bands
摘要用38 66不同复性温度处理,比较了18条随机引物的扩增结果.发现复性温度在40 50之间均有数量不等的扩增产物,不同引物的最高复性温度不同,有些引物用60以上的复性温度仍有扩增产物.一些在35 38的复性温度下非特异性产物较多的引物,通过大幅度提高复性温度,能提高扩增产物的特异性,获得清晰的rapd带型。 |
- Similar Words：
- "random posterior probability" English translation, "random posting" English translation, "random pressure" English translation, "random primed dna labeling system" English translation, "random primed dna labelling" English translation, "random primer extension" English translation, "random primer labelling" English translation, "random priority hunting" English translation, "random probability samples" English translation, "random probing" English translation
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